GAPDH Monoclonal Antibody | E-AB-20032

GAPDH Monoclonal Antibody | E-AB-20032 | Gentaur UK, US & Europe Distribution

Type: Control Antibody

Synonyms: Aging associated gene 9 protein, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, Peptidyl-cysteine S-nitrosylase GAPDH

Application: WB, IHC-p, IF

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Hamster, Pig, Rabbit, Sheep, Insect, Yeast

Host: Mouse

Isotype: IgG

Reserch Areas: Cancer, Metabolism, Signal Transduction

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. 

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Protein A purification

Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300

Clone: Clone:9S5

Conjugation: Unconjugated

Molecular Weight(Calculated): N/A

Molecular Weight(Observed): 37 kDa