GASP-1, human recombinant | 7152

Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF- binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.

7152 | GASP-1 human recombinant DataSheet

Biomolecule/Target: GASP-1

Synonyms: GDF-associated serum protein-1, GASP, KIAA0443, WFIKKNRP

Alternates names: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP

Taglines: A secreted inhibitory TGF- binding protein

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #:

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 ug/ml in the presence of 5ng/ml of human Myostatin (GDF-8)

Biological activity: 5 x 10 IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 6.5 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 7.8.

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long-term storage, it is recommend to add a carrier protein (e.g., 0.1% BSA). Prevent freeze/thaw cycles.

Amino acid sequence: N/A