GC Antibody (Center) [APR32624G]

GC Antibody (Center) [APR32624G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This GC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-365 amino acids of human GC.

Cellular Localisation: Lysosome membrane; Peripheral membrane protein; Lumenal side. Note=Interaction with saposin-C promotes membrane association (PubMed:10781797) . Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 (PubMed:18022370) .

Molecular Weight: 59716

Clone: Polyclonal

Gene Name: GBA (HGNC:4177)

Gene ID: 2629

Function: Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:9201993, PubMed:24211208, PubMed:15916907) . Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698) . Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation (PubMed:19279011) . Also plays a role in cholesterol metabolism (PubMed:24211208, PubMed:26724485) . May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208, PubMed:26724485) . The short chain saturated C8:0- GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (PubMed:24211208) . Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485) . Finally, may also hydrolyze cholesteryl- beta-D-glucoside to produce D-glucose and cholesterol (PubMed:24211208, PubMed:26724485) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC, IHC-P-Leica

Dilution: FC--1:25 WB--1:2000

Synonyms: Glucosylceramidase, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-GC, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase, GBA, GC, GLUC