GCLC polyclonal Antibody | BS90566

GCLC polyclonal Antibody | BS90566 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Application: WB IHC FC

Application Range: WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100

Background: The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC) . g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. 

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GCLC polyclonal Antibody detects endogenous levels of GCLC protein.

Molecular Weight: 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: GCLC, GLCL, GLCLC, EC 6.3.2.2, Gamma ECS, Gamma glutamylcysteine synthetase, Gamma-ECS antibod, Gamma-glutamylcysteine synthetase, GCL, Gclc, GCS, GCS heavy chain, GLCL, GLCLC, Glutamate cysteine ligase catalytic subunit, Glutamatecysteine ligase catalytic subunit, GSH1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: