GCN2 Antibody (N-term G11) [AMM08571G]

GCN2 Antibody (N-term G11) [AMM08571G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This GCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCN2.

Cellular Localisation: Cytoplasm {ECO:0000250|UniProtKB:Q9QZ05}.

Molecular Weight: 186911

Clone: Polyclonal

Gene Name: EIF2AK4 (HGNC:19687)

Gene ID: 440275

Function: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545) . Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity) . EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR- specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (By similarity) . Binds uncharged tRNAs (By similarity) . Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367) . Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity) . Plays a role in neurite outgrowth inhibition (By similarity) . Plays a proapoptotic role in response to glucose deprivation (By similarity) . Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress- activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity) . Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity) .

Summary: Tissue Location: Widely expressed (PubMed:10504407) . Expressed in lung, smooth muscle cells and macrophages (PubMed:24292273)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, EIF2AK4, GCN2, KIAA1338