GDA, human recombinant | 7807

GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7807 | GDA human recombinant DataSheet

Biomolecule/Target: GDA

Synonyms: Guanine deaminase, CYPIN, GUANASE, NEDASIN

Alternates names: Guanine deaminase, CYPIN, GUANASE, NEDASIN

Taglines: An enzyme responsible for the hydrolytic deamination of guanine.

NCBI Gene ID #: 25444

NCBI Gene Symbol: FGF9

Gene Source: Human

Accession #: P36364

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 0.9 unit/ml, in which one unit will deaminate 1.0 µmole of guanine to xanthine per minute at pH 8.0 at 25ºC.

Results: Specific activity is > 0.9 unit/ml

Binding Capacity: N/A

Unit Definition: One unit will deaminate 1.0 µmole of guanine to xanthine per minute at pH 8.0 at 25ºC.

Molecular Weight: 53 kDa (477 aa, 1-454 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGelG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV