General BSA (Bovine Serum Albumin) ELISA Kit | ELK1042

General BSA (Bovine Serum Albumin) ELISA Kit | ELK1042 | Gentaur UK, US & Europe Distribution

Alternative Names: Bovine Albumin; Fraction V

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 161.2 ng/mL

Standard: 30000 ng/mL

Detection Range: 468.75-30000 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Immune molecule

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Bovine Serum Albumin (BSA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bovine Serum Albumin (BSA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bovine Serum Albumin (BSA) in the samples is then determined by comparing the OD of the samples to the standard curve.