General CML (Carboxymethyl Lysine) ELISA Kit | ELK7896

General CML (Carboxymethyl Lysine) ELISA Kit | ELK7896 | Gentaur UK, US & Europe Distribution

Alternative Names: N (6) -Carboxymethyllysine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 25.9 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Genetic science;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carboxymethyl Lysine (CML) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carboxymethyl Lysine (CML) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxymethyl Lysine (CML) in the samples is then determined by comparing the OD of the samples to the standard curve.