General CNCbl (Cyanocobalamin) ELISA Kit | ELK7871

General CNCbl (Cyanocobalamin) ELISA Kit | ELK7871 | Gentaur UK, US & Europe Distribution

Alternative Names: VB12; Vitamin B12; Cobalamin; α-5, 6-Dimethylbenzimidazolyl Cobamidcyanide

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 49.2 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyanocobalamin (CNCbl) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyanocobalamin (CNCbl) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyanocobalamin (CNCbl) in the samples is then determined by comparing the OD of the samples to the standard curve.