General DHEA (Dehydroepiandrosterone) ELISA Kit | ELK7826

General DHEA (Dehydroepiandrosterone) ELISA Kit | ELK7826 | Gentaur UK, US & Europe Distribution

Alternative Names: DHEA

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 256 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dehydroepiandrosterone (DHEA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dehydroepiandrosterone (DHEA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dehydroepiandrosterone (DHEA) in the samples is then determined by comparing the OD of the samples to the standard curve.