General DHEA-S (Dehydroepiandrosterone sulfate) ELISA Kit | ELK8370

General DHEA-S (Dehydroepiandrosterone sulfate) ELISA Kit | ELK8370 | Gentaur UK, US & Europe Distribution

Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.25 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dehydroepiandrosterone sulfate (DHEA-S) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dehydroepiandrosterone sulfate (DHEA-S) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dehydroepiandrosterone sulfate (DHEA-S) in the samples is then determined by comparing the OD of the samples to the standard curve.