General DHVD3 (1, 25-Dihydroxyvitamin D3) ELISA Kit | ELK8091

General DHVD3 (1, 25-Dihydroxyvitamin D3) ELISA Kit | ELK8091 | Gentaur UK, US & Europe Distribution

Alternative Names: DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1, 25- (OH) 2D3; 1, 25-Dihydroxycholecalciferol; 1, 25-Dihydroxy Vitamin D3

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 49.5 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1, 25-Dihydroxyvitamin D3 (DHVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1, 25-Dihydroxyvitamin D3 (DHVD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1, 25-Dihydroxyvitamin D3 (DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.