General fT4 (Free Thyroxine) ELISA Kit | ELK3119

General fT4 (Free Thyroxine) ELISA Kit | ELK3119 | Gentaur UK, US & Europe Distribution

Alternative Names: FTI

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.48 pg/mL

Standard: 90 pg/mL

Detection Range: 1.41-90 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Thyroxine (fT4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Thyroxine (fT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Thyroxine (fT4) in the samples is then determined by comparing the OD of the samples to the standard curve.