General HS (Heparan Sulfate) ELISA Kit | ELK8398

General HS (Heparan Sulfate) ELISA Kit | ELK8398 | Gentaur UK, US & Europe Distribution

Alternative Names: HS; Heparan Sulfate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.19 ng/mL

Standard: 20 ng/mL

Detection Range: 0.31-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Heparan Sulfate (HS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparan Sulfate (HS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate (HS) in the samples is then determined by comparing the OD of the samples to the standard curve.