General HVA (Homovanillic Acid) ELISA Kit | ELK7921

General HVA (Homovanillic Acid) ELISA Kit | ELK7921 | Gentaur UK, US & Europe Distribution

Alternative Names: HOC6H3 (OCH3) CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.97 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Homovanillic Acid (HVA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Homovanillic Acid (HVA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Homovanillic Acid (HVA) in the samples is then determined by comparing the OD of the samples to the standard curve.