General HVD3 (25-Hydroxyvitamin D3) ELISA Kit | ELK7890

General HVD3 (25-Hydroxyvitamin D3) ELISA Kit | ELK7890 | Gentaur UK, US & Europe Distribution

Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH) D

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3 (HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3 (HVD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3 (HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.