General IP3 (Inositol Triphosphate) ELISA Kit | ELK8148

General IP3 (Inositol Triphosphate) ELISA Kit | ELK8148 | Gentaur UK, US & Europe Distribution

Alternative Names: InsP3; Inositol 1, 4, 5-Trisphosphate; Triphosphoinositol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 49.3 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inositol Triphosphate (IP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Triphosphate (IP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Triphosphate (IP3) in the samples is then determined by comparing the OD of the samples to the standard curve.