General LA (Lactic Acid) ELISA Kit | ELK8217

General LA (Lactic Acid) ELISA Kit | ELK8217 | Gentaur UK, US & Europe Distribution

Alternative Names: LA

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 2.92 μg/mL

Standard: 600 μg/mL

Detection Range: 9.38-600 μg/mL

Sample Type: serum, plasma and biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Cardiovascular biology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Lactic Acid (LA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lactic Acid (LA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactic Acid (LA) in the samples is then determined by comparing the OD of the samples to the standard curve.