General LTB4 (Leukotriene B4) ELISA Kit | ELK7887

General LTB4 (Leukotriene B4) ELISA Kit | ELK7887 | Gentaur UK, US & Europe Distribution

Alternative Names: LT-B4

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 89.3 pg/mL

Standard: 20000 pg/mL

Detection Range: 312.5-20000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Leukotriene B4 (LTB4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukotriene B4 (LTB4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukotriene B4 (LTB4) in the samples is then determined by comparing the OD of the samples to the standard curve.