General PEG (Polyethylene Glycol) ELISA Kit | ELK8264

General PEG (Polyethylene Glycol) ELISA Kit | ELK8264 | Gentaur UK, US & Europe Distribution

Alternative Names: PEO; POE; Polyethylene oxide; Polyoxyethylene; Carbowax; GoLYTELY; GlycoLax; Fortrans; TriLyte; Colyte; Halflytely; Macrogol; MiraLAX; MoviPrep

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.72 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: biological agents

Assay Lenght: 2h

Research Area: Gastrointestinal science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Polyethylene Glycol (PEG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polyethylene Glycol (PEG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polyethylene Glycol (PEG) in the samples is then determined by comparing the OD of the samples to the standard curve.