General PGF2a (Prostaglandin F2 Alpha) ELISA Kit | ELK7850

General PGF2a (Prostaglandin F2 Alpha) ELISA Kit | ELK7850 | Gentaur UK, US & Europe Distribution

Alternative Names: PGF2A; PGF2-A; PG-F2a

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 3.88 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin F2 Alpha (PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin F2 Alpha (PGF2a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin F2 Alpha (PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve.