General PIP3 (Phosphatidylinositol Trisphosphate) ELISA Kit | ELK8250

General PIP3 (Phosphatidylinositol Trisphosphate) ELISA Kit | ELK8250 | Gentaur UK, US & Europe Distribution

Alternative Names: PtdIns (3, 4, 5) P3

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 2.83 ng/mL

Standard: 500 ng/mL

Detection Range: 7.82-500 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylinositol Trisphosphate (PIP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylinositol Trisphosphate (PIP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol Trisphosphate (PIP3) in the samples is then determined by comparing the OD of the samples to the standard curve.