General PP (Dipalmitin) ELISA Kit | ELK8238

General PP (Dipalmitin) ELISA Kit | ELK8238 | Gentaur UK, US & Europe Distribution

Alternative Names: 1, 3-Dipalmitin; 1, 3-Di- (Hexadecanoyl) -Glycerol; 1, 3-Dipalmitoylglycerol; 1, 3-Dipalmitoyl-Glycerol, Glyceryl 1, 3-Dipalmitate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 4.94 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: biological agents

Assay Lenght: 2h

Research Area: Enzyme & Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dipalmitin (PP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dipalmitin (PP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipalmitin (PP) in the samples is then determined by comparing the OD of the samples to the standard curve.