General T4 (Thyroxine) ELISA Kit | ELK1204

General T4 (Thyroxine) ELISA Kit | ELK1204 | Gentaur UK, US & Europe Distribution

Alternative Names: 3, 5, 3', 5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.42 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine (T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine (T4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine (T4) in the samples is then determined by comparing the OD of the samples to the standard curve.