General TXB2 (Thromboxane B2) ELISA Kit | ELK7860

General TXB2 (Thromboxane B2) ELISA Kit | ELK7860 | Gentaur UK, US & Europe Distribution

Alternative Names: TX-B2

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 7.2 pg/mL

Standard: 1500 pg/mL

Detection Range: 23.44-1500 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thromboxane B2 (TXB2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thromboxane B2 (TXB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thromboxane B2 (TXB2) in the samples is then determined by comparing the OD of the samples to the standard curve.