General VD2 (Vitamin D2) ELISA Kit | ELK7870

General VD2 (Vitamin D2) ELISA Kit | ELK7870 | Gentaur UK, US & Europe Distribution

Alternative Names: Ergocalciferol; Deltalin; Drisdol; Calcidol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.53 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Metabolic pathway;Neuro science;Nutrition metabolism;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D2 (VD2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D2 (VD2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D2 (VD2) in the samples is then determined by comparing the OD of the samples to the standard curve.