GFP | Green Fluorescence Protein (total IgG) | AS15 2999

GFP | Green Fluorescence Protein (total IgG) | AS15 2999 | Gentaur UK, US & Europe Distribution

Immunogen: highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG in 10 mM TRIS, pH 7.2. Contains 0.02 % sodium azide.

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: AS15 2987 | Anti-GFP (affinity purified), rabbit antibodiesAS15 3000 | Anti-GFP (protein A purified), rabbit antibodiesAS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodiesAS15 2998 | Anti-GFP (total IgY), chicken antibodíesAS15 3002 | GFP (purified recombinant protein) other tag antibodies

Recommended Dilutions: 1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Native GFP, Recombinant GFP (E, coli), all variants of GFP

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: Minimal cross-reactivity with E.coli proteins

Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: GFP/YFP/RFP/CFP

Research Area: Tag Antibodies