GFP Stably Expressing Jurkat Cell Line | T3920

GFP Stably Expressing Jurkat Cell Line | T3920

GFP Stably Expressing Jurkat Cell Line

Biosafety:

II

Organism:

Human

Source Organ:

Peripheral Blood

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

40 - 50 hours

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Puromycin

Applications:

N/A

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 1500rpm for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO2.

Propagation:

The base medium for this cell line is PriGrow II medium available at abm

Preservation:

N/A

Quality Control:

1. Puromycin selection 2. FACS Sorting of very high GFP expressing population.

Tumorgenicn:

yes

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C