Glucokinase, human pancreatic, recombinant | 7777

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

7777 | Glucokinase human pancreatic recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: N/A

Alternates names: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D

Taglines: Plays an important role in the regulation of carbohydrate metabolism

NCBI Gene ID #: 27006

NCBI Gene Symbol: FGF-22

Gene Source: Human pancreas

Accession #: Q9HCT0

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30oC for 30 min.

Molecular Weight: 53.1 kDa

Concentration: 39.3 mg/ml

Appearance: Liquid

Physical form description: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A