GLUD1 / GLUD2 Antibody | 42-524

GLUD1 / GLUD2 Antibody | 42-524 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat

Homology: Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen: The immunogen for this antibody is: C-ESEEQKRNRVRGILR

Research Area: Neuroscience, Signal Transduction

Tested Application: E, WB, IHC, IF

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1) . Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5ug/ml.Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2) .

Positive Control 1: Cat. No. 1304 - Human Liver Tissue Lysate

Positive Control 2: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003, glutamate dehydrogenase (NAD (P) +) , GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1

User Note: N/A

BACKGROUND: N/A