GLuR5 Antibody | 45-679

GLuR5 Antibody | 45-679 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat

Immunogen: The immunogen for this antibody is: QCKQTHPTNSTS

Research Area: Cancer

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al, J Neurosci. 2004 Oct 13;24 (41) :8986-93.; PMID: 15483117) . Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 3.75ug/ml.

Specificiy: This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.

Positive Control 1: Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3, EEA3, GLR5, excitatory amino acid receptor 3

User Note: N/A

BACKGROUND: N/A