Glutamate Decarboxylase α (GAD α), E. coli Recombinant | P1404

Glutamate Decarboxylase α is a pyridoxal 5′-phosphate dependent enzyme. GAD α catalyzes conversion of glutamate to GABA. Aberrant expressions of GAD α have been associated with diabetes and neurodegenerative diseases such as Alzheimers and Parkinson’s Disease.

P1404 | Glutamate Decarboxylase α (GAD α), E. coli Recombinant DataSheet

Biomolecule/Target :

Synonyms: GAD-alpha, gadA, gadS

Alternates names: GAD-alpha, gadA, gadS

Taglines: Converts glutamate to gamma-aminobutyrate (GABA)

NCBI Gene ID #: 948027

NCBI Gene Symbol: gadA

Gene Source: E Coli

Accession #: P69908

Recombinant: True

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): This enzyme has a specific activity of ≥ 1 mU/mg based on its ability to convert glutamate to GABA, which can be detected fluorometrically at Ex/Em=535/595.

Biological activity:

Results: 1 mU/mg

Binding Capacity:

Unit Definition: One unit is the amount of enzyme that will hydrolyze 1.0 umole of glutamate per minute at pH 8 and 37 ̊C.

Molecular Weight: 53.6 kDa with C-terminal 6X-His tag

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute the lyophilized protein in 10% glycerol with 5 mM 2-Mercaptoethanol to a final concentration of 1.0 mU/ml and incubate the reconstituted protein at 25 °C for 15 minutes.

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: