Glyoxalase II (Glx II), Human Recombinant | P1072

The glyoxalase system consists of Glyoxalase I (Glx I) and Glyoxalase II (Glx II) which is responsible for detoxifying -ketoaldehydes such as the potent and cytotoxic methylglyoxal (MG). Glx I converts MG to S-D-lactoyl-glutathione (SLG). SLG is then hydrolyzed to D-lactate and reduced glutathione by Glx II. Glx II desensitizes cells to p53-dependent DNA damage-induced apoptosis. It also plays a role during development and in the pathogenesis of cancer and neurodegenerative diseases. Glyoxalase II may also be important in the regulation of spermatogenesis.

P1072 | Glyoxalase II Glx II Human Recombinant DataSheet

Biomolecule/Target: HAGH

Synonyms: Hydroxyacylglutathione hydrolase, mitochondrial, HAGH1, GLO2

Alternates names: Connective Tissue Growth Factor, CCN2, Hypertrophic chondrocyte-specific protein 24 (HCS24), Insulin-like growth factor-binding protein 8

Taglines: Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

NCBI Gene ID #: 1490

NCBI Gene Symbol: CTGF

Gene Source: Human

Accession #: NM_001901

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ED 1.0–2.0 g/ml determined by the dose-dependent stimulation of the proliferation of HUVEC cells.

Results: 10 U/mg

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that will hydrolyze 1.0 µmole of SLG per minute at pH 7.4 at 25 C.

Molecular Weight: 11.2 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 30% glycerol, 50 mM Tris pH 7.4 and 150 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A