GNA13 Antibody (Center) [APR16176G]

GNA13 Antibody (Center) [APR16176G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This GNA13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human GNA13.

Cellular Localisation: Cell membrane; Lipid-anchor. Melanosome. Cytoplasm. Nucleus Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) . Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages (PubMed:18703424) . In round spermatids, also present in the nuclei (PubMed:18703424) .

Molecular Weight: 44050

Clone: Polyclonal

Gene Name: GNA13

Gene ID: 10672

Function: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452) . Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866) . GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity) . Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452) . Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333) .

Summary: Tissue Location: Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level) . Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Guanine nucleotide-binding protein subunit alpha-13, G alpha-13, G-protein subunit alpha-13, GNA13