GNPDA1 Antibody | 5049

GNPDA1 Antibody | 5049 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.
The immunogen is located within the last 50 amino acids of GNPDA1.

Research Area: Homeostasis

Tested Application: E, WB, ICC, IF

Application: GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: GNPDA1 is predicted to not cross-react with GNPDA2.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: GNPDA1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GNPDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: GNPDA1 Antibody: GPI, HLN, GNP1, GNPI, GNPDA, KIAA0060, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.