GnRH ELISA Kit | E5099-100

GnRH ELISA Kit | E5099-100

Description: Gonadotropin-releasing hormone (GnRH) is produced and secreted by specialized nerve cells in the hypothalamus of the brain. It is released into tiny blood vessels that carry this hormone from the brain to the pituitary gland. GnRH is a releasing hormone responsible for the release of follicle-stimulating hormone and luteinizing hormone from the anterior pituitary. These hormones are released into the general circulation and act on the testes and ovaries to initiate and maintain their reproductive functions. Treatment with gonadotropin-releasing hormone agonists of uterine myoma, endometriosis and some hormone-dependent cancers, such as breast, ovarian, endometrial and prostate cancer, also seems to have a beneficial effect. ’s GnRH ELISA Kit is used to quantitatively measure GnRH in Serum, plasma and other biological fluids. The kit is based on the Competitive ELISA principle. The micro-ELISA plate provided in this kit has been preoated with GnRH. During the reaction, GnRH in samples or Standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GnRH in the samples is calculated by comparing the OD of the samples to the standard curve.

Alternate Name: Gonadotropin Releasing Hormone ELISA Kit; GnRH ELISA Kit; GNRH1 ELISA Kit; GNRH ELISA Kit; GRH ELISA Kit; HH12 ELISA Kit; LHRH ELISA Kit; LNRH ELISA Kit; gonadotropin releasing hormone 1 ELISA Kit

Tag Line: A Competitive ELISA kit for in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Serum, plasma and other biological fluids

Species Reactivity: All

Applications: The ELISA kit is used for in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

Features & Benefits: Detection Range: 15.63-1000 pg/ml Sensitivity: 9.38 pg/ml Highly sensitive and specific