Goat anti-Dog IgG (H&L), Unconjugated | AS16 3124

Goat anti-Dog IgG (H&L), Unconjugated | AS16 3124 | Gentaur UK, US & Europe Distribution

Immunogen: purified dog normal IgG isolated from pooled dog serum

Host: Goat

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Protein G purified goat IgG in PBS pH 7.2.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP)

Related Products: other unconjugated secondary antibodies to dog IgGSecondary antibodies

Recommended Dilutions: Immunoprecipitation, In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells, Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration

Molecular weight: N/A

Confirmed Reactivity: Dog IgG (heavy & light chains)

Predicted Reactivity: N/A

Not reactive in: This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Additional Information: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.

Background: Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.

Reconstitution: For reconstitution add 1 ml of sterile destilled water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.

TAIR Nnumbre: N/A

Category: Unconjugated

Research Area: Immunoassays