Goat Anti-GFP IgG Polyclonal Antibody | AB0020

Goat Anti-GFP IgG Polyclonal Antibody | AB0020 | Antibodies

Overview: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Other Names: Green fluorescent protein antibody.

Other Names: Green fluorescent protein antibody.

Concentration: 2 mg/ml

Antibody Type: Primary

Target Type: anti-GFP

Accession Number: N/A

Host Species: Goat

Immunogen Species: N/A

Immunogen Type: Recombinant protein

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Clonality: Polyclonal

Confirmed Reactive Species: GFP

Specificity Detail: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Buffer: PBS, 20% glycerol and 0.05% sodium azide

Purification: Epitope affinity purified

Conjugation: Unconjugated

Format: N/A

Application: WB, IF, IHC-P, IHC-F

Concentration/Dilution: WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000

Storage: N/A

References: 1. Wu JS1, Yi E2, Manca M, et al. Elife 2020 Jan. PMID:31975688, 2. Carey LM, PhD Thesis, Indiana University, US 2019, 3. Fukuda S, Kaneshige A, Kaji T, et al. Elife 2019 Sep. PMID:31545169, 4. Matsuda T, Oinuma I. Sci Rep 2019 Aug. PMID:31383899, 5. Geller S, Arribat Y, Netzahualcoyotzi C, et al. Cell Metab 2019 Aug. PMID:31474567, 6. Zhang L, Uezumi A, Kaji T, et al. Int J Mol Sci 2019 Jul. PMID:31277245, 7. Alenquer M, Vale-Costa S, Etibor TA, et al. Nat Commun 2019 Apr. PMID:30967547, 8. Koike M, Yutoku Y, Koike A. FEBS Open Bio 2019 Jan. PMID:31115163, 9. Vyas P, Wu JS, Jimenez A, et al. Sci Rep 2019 Apr. PMID:30944354, 10. Alenquer M, Vale-Costa S, Sousa AL, et al. bioRxiv 410373; Sept 2018, 11. Larson VA, Mironova Y, Vanderpool KG, et al. Elife. 2018 Mar. PMID:29596047, 12. Festa BP, Chen Z, Berquez M, et al. Nat Commun 2018 Jan. PMID:29323117, 13. Ramos-Nascimento A, Kellen B, Ferreira F, et al. J Cell Sci 2017 Dec. PMID:29061883, 14. Wu JS, Vyas P, Glowatzki E, et al. J Comp Neurol 2017 Oct. PMID:29055051, 15. Agarwal A, Wu PH, Hughes EG, et al. Neuron 2017. PMID:28132831, 16. Mimura Y, Takagi M, Clever M, et al. J Cell Sci 2016 Nov. PMID:27802161, 17. Vyas P, Wu JS, Zimmerman A, et al. J Assoc Res Otolaryngol 2016 Sep. PMID:27696081, 18. Vale-Costa S, Alenquer M, Sousa AL, et al. J Cell Sci 2016 Mar. PMID:26940915, 19. Martins-Marques T, Anjo SI, Pereira P et al. Mol Cell Proteomics 2015 Aug. PMID:26316108 Supplemental Information, 20. Sargiannidou I, Kim GH, Kyriakoudi S et al. Neurogenetics 2015 Jul. PMID: 25771809, 21. Kourti M, Ikonomou G, Giakoumakis NN, et al. Cell Signal 2015 Mar. PMID: 25744540, 22. Monteiro NCO, MSc Thesis, University of Coimbra, Portugal 2015, 23. Ferreira RRS, MSc Thesis, University of Coimbra, Portugal 2015, 24. Paukert M, Agarwal A, Cha J, et al. Neuron 2014 Jun. PMID: 24945771 Supplemental Information, 25. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014, 26. Pais SV, Milho C, Almeida F, et al. PLoS One 2013. PMID: 23431368