Goat Anti-Mouse IgG (H&L) Antibody (HRP) | XR-9720

Goat Anti-Mouse IgG (H&L) Antibody (HRP) | XR-9720 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Mouse

Immunogen: Mouse IgG - whole molecule

Tested Application: E, WB, IHC

Application: Peroxidase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot) , ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:50,000 - 1:200,000, Western Blot 1:4,000 - 1:20,000, Immunohistochemistry 1:1,000 - 1:8,000. (Optimal dilution should be determined by user) .

Specificiy: Minimum Cross reactivity to Human Serum Proteins

Purification: Peroxidase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.

Isotype: IgG

Conjugate: HRP

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA) ; 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Concentration: N/A

Storage Condition: Peroxidase Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 ˚C prior to restoration. For extended storage aliquot contents and freeze at -20 ˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use.

Target Species: Reacts with the heavy chain of mouse IgG.