Goat Anti-Rat IgG (H&L) Antibody (HRP) | XR-9520

Goat Anti-Rat IgG (H&L) Antibody (HRP) | XR-9520 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Rat

Immunogen: Rat IgG - whole molecule

Tested Application: E, WB, IHC

Application: Peroxidase Conjugated Affinity Purified anti-Rat IgG is suitable for immunoblotting (western or dot blot) , ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:50,000 - 1:250,000, Western Blot 1:5,000 - 1:25,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user) .

Specificiy: Minimum Cross reactivity to Human Serum Proteins

Purification: Peroxidase Conjugated Affinity Purified anti-Rat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Isotype: IgG

Conjugate: HRP

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA) ; 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Concentration: N/A

Storage Condition: Peroxidase Conjugated Affinity Purified anti-Rat IgG can be stored vial at 4 ˚C prior to restoration. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 ˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use.

Target Species: Reacts with the heavy chain of rat IgG.