GOLGA5 Antibody | 7387

GOLGA5 Antibody | 7387 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5.
The immunogen is located within amino acids 540 - 590 of GOLGA5.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 64, 80 kDa
Observed: 65, 78 kDa

Validation: N/A

Isoform: N/A

Purification: GOLGA5 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GOLGA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5) , also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1) . It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1, 2) . As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3, 4) . Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5) .