GPT2/ALT2, human recombinant | 7806

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7806 | GPT2/ALT2 human recombinant DataSheet

Biomolecule/Target: GPT2/ALT2

Synonyms: Alanine aminotransferase 2, AAT2, ALT2

Alternates names: Alanine aminotransferase 2, AAT2, ALT2

Taglines: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate

NCBI Gene ID #: 56636

NCBI Gene Symbol: FGF-21

Gene Source: Human

Accession #: Q9JJN1

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 30 unit/mg; One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.

Results: Specific activity is > 30 unit/mg

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.

Molecular Weight: 60.3 kDa (546 aa, 1-523 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 7.5), 30% glycerol, 0.2 M NaCl and 2 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA