Granzyme B, human recombinant (Insect) | 4728

Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

4728 | Granzyme B human recombinant Insect DataSheet

Biomolecule/Target: N/A

Synonyms: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11

Alternates names: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11

Taglines: A serine protease involved in CTL mediated apoptosis

NCBI Gene ID #: 56636

NCBI Gene Symbol: FGF-21

Gene Source: Human

Accession #: Q9JJN1

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.

Results: 40 units/g

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 32 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized powder from 0.22 m filtered solution in PBS containing 8% trehalose

Reconstitution Instructions: Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.

Amino acid sequence: N/A