Granzyme B, Mouse recombinant (Insect) | 7608

Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and enters the target cell, assisted by perforin. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

7608 | Granzyme B Mouse recombinant Insect DataSheet

Biomolecule/Target: N/A

Synonyms: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, mouse lymphocyte protein, HLP, C11

Alternates names: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11

Taglines: An active serine protease which processes pro-caspases to induce apoptosis

NCBI Gene ID #: 56636

NCBI Gene Symbol: FGF-21

Gene Source: Mouse

Accession #: Q9JJN1

Recombinant: Yes

Source: Insect cell

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 40 units/mg

Biological activity: Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em = 380/500 nm.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl2.

Molecular Weight: 33 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: rmGranzyme is supplied as a lyophilized powder

Reconstitution Instructions: Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.

Amino acid sequence: N/A