GRB10 Antibody | 6733

GRB10 Antibody | 6733 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%)

Immunogen: GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10.
The immunogen is located within amino acids 440 - 490 of GRB10.

Research Area: Signal Transduction

Tested Application: E, WB, IF

Application: GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: GRB10 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GRB10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: GRB10 Antibody: RSS, IRBP, MEG1, GRB-IR, Grb-10, GRBIR, KIAA0207, Growth factor receptor-bound protein 10, GRB10 adapter protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.