GRK6 Antibody | 7641

GRK6 Antibody | 7641 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6.
The immunogen is located within the last 50 amino acids of GRK6.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, IHC-P, IF

Application: GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family.

Positive Control 1: Cat. No. 1468 - Rat Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 63 kDa
Observed: 63 kDa

Validation: N/A

Isoform: N/A

Purification: GRK6 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GRK6 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GRK6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: GRK6 Antibody: GPRK6, GPRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The G protein–coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1, 2) . Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1) . GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3, 4) , and the insulin-like growth factor signaling pathway (4) . GRK6 may also play a role in immune system function (5) .