GSK3α/β (Phospho-Y216/Y279) polyclonal Antibody | BS94064

GSK3α/β (Phospho-Y216/Y279) polyclonal Antibody | BS94064 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC IHC IP

Application Range: WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200

Background: Glycogen synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K) -protein kinase B (PKB) -dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GSK3α/β (Phospho-Y216/Y279) polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Tyr279/216

Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: GSK-3 alpha, GSK3A, GSK-3 beta, GSK3B, Factor A, Glycogen synthase kinase 3 alpha, Glycogen synthase kinase 3 beta, GSK3 alpha, GSK3 beta, GSK3B,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3 (alpha+beta) .

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: ProA affinity purified

Pathway: