GSK3α/β (phospho-Y279/216) polyclonal Antibody | BS4083

GSK3α/β (phospho-Y279/216) polyclonal Antibody | BS4083 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:2000 IHC: 1:50~1:200

Background: Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase) -protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-GSK3α/β (Y279/216) polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Tyr279/216

Molecular Weight: ~ 46,51 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Immunogen: Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Tyrosine 279.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: PI3K AKT signaling,Hedgehog Signaling,Hypoxia Signaling,Translational Contral Regulation of elF2,Regulation of Microtubule Dynamics,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,MTOR signaling,