GST-tag (rabbit antibody, polyclonal) | AS17 4147

GST-tag (rabbit antibody, polyclonal) | AS17 4147 | Gentaur UK, US & Europe Distribution

Immunogen: GST protein

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1: 1: 1000 (ELISA), 1: 2000 (WB)

Molecular weight: 26 kDa

Confirmed Reactivity: N/A

Predicted Reactivity: native and denatured forms of purified GST or GST fusion proteins

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Overnight incubation of this antibody with dilution 1:5 000 with 0, 5 µg of GST-tagged protein produced strong background but works fine with samples of total Escherichia coli lysate if GST-tagged protein is expressed in low amounts.

Background: GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: GST

Research Area: Tag Antibodies