Guinea pig RANTES (Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit | ELK5820

Guinea pig RANTES (Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit | ELK5820 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 5

Species: Guinea pig

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in the samples is then determined by comparing the OD of the samples to the standard curve.